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Image Search Results
Journal: Bioactive materials
Article Title: Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy.
doi: 10.1016/j.bioactmat.2021.07.012
Figure Lengend Snippet: Fig. 5. Functional activity of havPD-1 EVs in cell-based assays. (a) Neutralization of PD-L1 in a cell assay by havPD-1 EVs in comparison with a clinical control, Atezolizumab. (b) Mixed lymphocyte reaction assay. Supernatants were measured for IFN-γ production by ELISA (n = 8). (c) Tetanus toxoids recall assay. Super natants were measured for IFN-γ production by ELISA (n = 8) (d) Antigen-dependent cell-mediated cytotoxicity assay (n = 8). (e) Complement dependent cyto toxicity assay (n = 8).
Article Snippet: After incubation for 3 days at 37 ◦C and 5% CO2, supernatants were harvested and subjected to detection of
Techniques: Functional Assay, Activity Assay, Neutralization, Comparison, Control, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay
Journal: Journal of Medical Virology
Article Title: Seronegative Vaccinees May Not Benefit From Multiple Booster Doses of MMR Vaccine in Restoring Immunity
doi: 10.1002/jmv.70135
Figure Lengend Snippet: Representation of normalized parameters related to immune CD4 + (a, b) and CD8 + (c, d) T cell populations before and after stimulation with UV‐inactivated measles (a, c) or rubella (b, d) virus with relative IFN‐γ induction (e, f). Nonstimulated samples (blue) are linked to the stimulated counterparts (measles, red; rubella, green) through connection lines. Among both populations, Naïve, Central Memory (CM), Effector Memory (EM), and Terminally Differentiated Effector Memory (TEMRA) cells were analyzed. Moreover, among CD4 + cells, T helper (Th) 1, 2, and 17 were analyzed. Results were expressed as the variation in the population percentage (with respect to CD4 + and CD8 + cells). Statistical significance was assessed using the two‐tailed χ 2 test. The p values are reported in the figures, where * stands for p < 0.05 and ** for p < 0.01. No differences were reported in the CD4 + and CD8 + populations, as well as in IFN‐γ induction, between the nonstimulated and measles‐stimulated groups.
Article Snippet: To further evaluate both T and NK cell response following stimulation with UV‐inactivated measles and rubella viruses (the latter serving as a positive control), supernatants of stimulated PBMCs were analyzed for IFN‐γ and Granzyme B expression using the
Techniques: Virus, Two Tailed Test